Isolation and Characterization of a HpyC1I Restriction-Modification System in Helicobacter pylori
نویسندگان
چکیده
منابع مشابه
Comparative genomics of the restriction-modification systems in Helicobacter pylori.
Helicobacter pylori is a Gram-negative bacterial pathogen with a small genome of 1.64-1.67 Mb. More than 20 putative DNA restriction-modification (R-M) systems, comprising more than 4% of the total genome, have been identified in the two completely sequenced H. pylori strains, 26695 and J99, based on sequence similarities. In this study, we have investigated the biochemical activities of 14 Typ...
متن کاملTranscriptional phase variation of a type III restriction-modification system in Helicobacter pylori.
Phase variation is important in bacterial pathogenesis, since it generates antigenic variation for the evasion of immune responses and provides a strategy for quick adaptation to environmental changes. In this study, a Helicobacter pylori clone, designated MOD525, was identified that displayed phase-variable lacZ expression. The clone contained a transcriptional lacZ fusion in a putative type I...
متن کاملPhenotypic and genotypic variation in methylases involved in type II restriction-modification systems in Helicobacter pylori.
To determine relationships between Helicobacter pylori geographical origin and type II methylase activity, we examined 122 strains from various locations around the world for methylase expression. Most geographic regions possessed at least one strain resistant to digestion by each of 14 restriction endonucleases studied. Across all of the strains studied, the average number of active methylases...
متن کاملHelicobacter pylori and Migraine: Is Eradication of Helicobacter pylori Effective in Relief of Migraine Headache?
Background: Association between Helicobacter pylori (HP) infection and migraine and the effect of HP eradication on relief of migraine headache have been studied but the results are controversial. Objectives: To evaluate the effect of HP eradication in treatment of patients affected by migraine. Materials and Methods: Eighty consecutive HP infected patients affected by migraine without au...
متن کاملCloning and characterization of the MboII restriction-modification system.
The two genes encoding the class IIS restriction-modification system MboII from Moraxella bovis were cloned separately in two compatible plasmids and expressed in E. coli RR1 delta M15. The nucleotide sequences of the MboII endonuclease (R.MboII) and methylase (M.MboII) genes were determined and the putative start codon of R.MboII was confirmed by amino acid sequence analysis. The mboIIR gene s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2004
ISSN: 0021-9258
DOI: 10.1074/jbc.m311639200